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KMID : 0381120070290040437
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Genes and Genomics
2007 Volume.29 No. 4 p.437 ~ p.445
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Genetic Differences and Variation in Black Rockfish (Sebastes schlegeli) and Hwanghae Rockfish (S. koreanus) from the Yellow Sea
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Yoon Jong-Man
Choi Youn
Kim Jong-Yeon
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Abstract
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Black rockfish (Sebastes schlegeli; BR) and Hwanghae rockfish (S. koreanus; HHR) were obtained from the Yellow Sea of Gunsan in Korea. In this study, seven oligonucleotide primers generated a total of 884 bands in the BR species, and 632 in the HHR species, with a DNA fragment size ranging from 150 to 2,200 bp. On average, a decamer primer generated 126.3 amplified products in the BR species. A RAPD primer generated an average of 11.5 amplified bands per sample, ranging from 7.3 to 15.5 bands in this rockfish species. In the HHR species, shared unique bands, corresponding to bands of 230 bp, 750 bp, 800 bp and 1,600 bp, were generated by the decamer primer, OPA-16. Seven primers generated 75 polymorphic bands (75/884 bands, 8.48%) in the BR species and 62 (62/632 bands, 9.81%) in the HHR species. The percentage of polymorphic bands obtained from the BR species varied from 7.2% to 9.9%. Seven primers were the highest levels of polymorphism, which was 12.9%, in the HHR species. The 180 bp and 200 bp bands produced by the primer OPB-14 were identified as being unique to individual BR species. Individuals of the BR species exhibited comparatively higher bandsharing values than did fish of the HHR. The average bandsharing values were approximately 0.838 ± 0.004 and was 0.690 ± 0.012 in the BR and HHR species, respectively. The average bandsharing value between the two rockfish species
was approximately 0.527 ± 0.005. The dendrogram, generated by seven reliable primers, indicates three genetic clusters. To distinguish the species of rockfish, our data suggested that the molecular marker based differentiation method could be a better than the traditional morphological character based one.
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KEYWORD
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black rockfish, dendrogram, genetic distance, Hwanghae rockfish, Sebastes koreanus
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Listed journal information
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